SOCS2, ID (Suppressor of Cytokine Signaling 2, SOCS-2, Cytokine-inducible SH2 Protein 2, CIS-2, STAT-induced STAT Inhibitor 2, SSI-2, CIS2, SSI2, STATI2)

Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous aa long sequence at the C-terminus that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-2 is 198aa long with short N-terminus and shares sequence similarity with CIS. SOCS-2 mRNA normally presents at very low levels in different tissues; however, its level is rapidly increased upon stimulation with IL-1, IL-3, IL-4, IL-6, LIF, GM-CSF, G-CSF, EPO, IFNg, prolactin, and GH. Similar to CIS, SOCS-2 does not bind to Janus- activated kinases; however, it can associate with GH, IGF-1, prolactin and leptin receptors. For example, SOCS-2 binds to Y1077 motif of leptin receptor and thus acts as a negative regulator of STAT5.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S1040-02M

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hilton D. J. et al. (1998) Biochemistry 195: 114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15 (11): 1493-1504. 4. Cooney R.N. (2002) Shock 17(2): 83-90. 5. Lavens D. (2006) J. of Cell Sc. 119: 2214-2224. 6. GoldshmitGoldshmit Y. et al. (2004) J. of Biol. Chem. 279(16):16349-16355.

Additional Info

Recognizes human SOCS-2. Species Crossreactivity: rat and mouse.