Sodium-dependent Multi-Vitamin Transporter (SMVT)

A sodium-dependent transport system is responsible for the transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2 and Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc. Sodium-dependent Multi-Vitamin Transporter (SMVT), a 635aa protein in rat and human (gene SLC5A6) is responsible for transplacental transfer of vitamins pantothenate, biotin and the essential metabolite lipoate. SMVT shows homology to other known sodium-dependent nutrient transporters, including bacterial pantothenate permease, mammalian iodide transporter, glucose transporter 1 & 2. Quantitatively, the absorptive tissues like the intestinal mucosa, kidney and placenta have very high amounts of SMVT-specific mRNA. Significant amount is also seen in liver, brain and heart.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml\nWestern Blot: 1-10ug/ml (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5327-25

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstititue with 100ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wang H et al (1999) JBC 275 (21), 14875-83. 2. Chatterjee, N. S (1999) Submitted Research Service, VA Med Center, Long Beach, CA. 3. Balamurugan K, 2003, Am J Physiol Renal Physiol, 285:73-77. 4. Nabokina SM, 2003, Am J Physiol Renal Physiol, 284: 737-742.

Additional Info

Recognizes rat SMVT.