Sodium-dependent Vitamin C Transporter 1 (SVCT1)

A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc. Sodium-dependent Vitamin C Transporter (SVCT), Vitamin C is now known to mediate a variety of enzymatic reactions, including collagen synthesis, the basis for the defect in scurvy, the vitamin also protects tissues from oxidative damage by scavenging free radical. The vitamin C absorption and distribution requires SVCT1 and SVCT2. SVCT1 605aa protein in rat, 604 in human (chrm 5, gene SLC23A1) contain up to 12 transmembrane domains, two possible sites for N-glycosylation and multiple phosphorylation sites. It is 65% identical to SVCT2 and largely expressed in epithelial surfaces involved in bulk transport such as intestine, liver and kidney.\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml; S5327-40A Control peptide can be used to coat ELISA plates at 1ug/ml. \nWestern Blot: 1-10ug/ml using ECL technique.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5327-40

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sotiriou, S et al (2002) Nature Med 8, 514; 2. Tsukaguchi, H et al (1999) Nature 399, 70; 3. Rajan, DP et al (1999) BBRC 262, 762; 4. Faaland, CA et al (1998) BBA 1442, 353; 5. Nagase, T et al (1996) DNA Res 3, 321; 6. Warden CH et al (1993) Genomics 18, 295.

Additional Info

Recognizes human SVCT1. No significant homology exist with SVCT2 or other related transporters. Species Sequence Homology (40-45%): Mouse, rat.