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Sphingomyelin Colorimetric Assay Kit

Cat no: 10009928

Sphingomyelin Colorimetric Assay Kit

Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman's SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.

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SPECIFICATIONS

Catalog Number

10009928

Form

96 Well

P Type

Assay Kits|Ceramides/Sphingolipids|Lipids|Small Molecule Detection (Non-EIA)

Weight

0

Storage Temp

4

Shipping Temp

4

Additional Info

Cayman's sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.

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