Sphingomyelinase Inhibitor Screening Assay Kit

Cayman's Sphingomyelinase Inhibitor Screening Assay provides a convenient method for screening sphingomyelinase inhibitors. This assay includes a neutral bacterial SMase. Even though bacterial SMase only shares 20% sequence homology with mammalian neutral SMase, it has been shown to share a common catalytic site as the mammalian SMase and similar inhibition profile. Cleavage of the sphingomyelin conjugate by SMase results in the release of a ceramide analog containing a free thiol which is detected by the fluorescent SMase Detector. This fluorescence is analyzed with an excitation wavelength between 375-385 nm and an emission wavelength between 510-520 nm.

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SPECIFICATIONS

Catalog Number

700330

Form

96 Well

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Sphingomyelinase (SMase) is a hydrolase enzyme that is involved in sphingolipid metabolism. SMase is a member of the DNase I superfamily of enzymes and is responsible for the breakdown of sphingomyelin into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stress. SMases are important in many physiological and pathophysiological processes, including: 1. lysosomal digestion of sphingomyelin, which is important for normal neuronal and vascular function; 2. ceramide-mediated signal transduction, leading to cytokine-induced apoptosis, cellular differentiation, and various immune and inflammatory responses; 3. lipoprotein aggregation within the vessel wall, which is a key event in atherogenesis; and 4. intracellular cholesterol trafficking and metabolism. Finding inhibitors to SMase (both acidic and neutral enzymes) could be beneficial in determining agents which reduce SMase activity and ceramide levels leading to attenuation of apoptosis and cellular proliferation, possible anti-depressant effects in depressive disorders, and beneficial clinical effects in acute or chronic neurodegenerative disorders, such as stroke and Alzheimer