Sphingosine Kinase 1 Polyclonal FITC Antibody

Antigen: human SPHK 1 amino acids 264-274 . Host: rabbit . Cross Reactivity: (+) human and mouse SPHK 1; expected to react with rat SPHK 1 . Application(s): FC, IF, and WB . SPHK 1 catalyzes the phosphorylation of sphingosine to S1P, a key lipid mediator that plays an important role in determining cell proliferation versus cell death.

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SPECIFICATIONS

Catalog Number

10012201

Applications

FC, IF, WB

Hosts

Rabbit

Form

1 ea

P Type

Antibodies|Intracellular Signaling

Weight

0

Antigen

human SPHK 1 amino acids 264-274

Storage Temp

-20

Shipping Temp

-20

Additional Info

The primary use of this antibody conjugate is for the detection of SPHK 1 in intact cells by direct immunolabeling methods such as flow cytometry or immunofluorescence microscopy. SPHK 1 is one of the enzymes involved in sphingolipid metabolism. SPHK 1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. This reaction plays an important role in determining cell proliferation versus cell death. SPHK 1 is found in a wide variety of tissues and cell types including kidney, liver, spleen, heart, platelets, and human tumors. On a cellular level, it is found in the cytosolic and membrane fractions. Based on the amino acid sequence, this protein has a molecular weight of approximately 43 kDa. Some reported post translational modifications may explain the shift in band migration to 50 kDa. NOTE: Multiple isoforms of SPHK 1 are known and one is 470 amino acids. This likely explains the 50 kDa band observed.