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Src, active, Recombinant, Human, GST-Tag (pp60Src, pp60c-Src)

Cat no: S6500-11A

Src, active, Recombinant, Human, GST-Tag (pp60Src, pp60c-Src)

Recombinant full-length human SRC with N-terminal GST tag (Gene accession number NM_005417), expressed in a Baculovirus infected Sf9 cell expression system.\n\nSpecific Activity: ~55 pmol/min/ug. \n\nAssay condition: 50mM HEPES (pH7.5), 10mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200uM ATP, 2uM Tyr2 substrate(Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature. \n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

S6500-11A

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.

Purity

~60%

References

1. Collett, M S. et al. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4.\n2. Jacobs, C. et al. Cancer Res. 1983 Apr;43(4):1696-702.

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