Src, phosphorylated (Tyr418) (pp60Src, pp60c-Src)

Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic SH2 domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This activity is negatively regulated when tyrosine 529 of Src is phosphorylated by Csk. This conformation holds Src in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilution to be determined by the researcher. \n\nPositive Control: \nChick Embryo Fibroblast (CEF) cells expressing human wild-type Src protein.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S6504-45R

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Ch/Bird

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Bock, H.H. and J. Herz (2003) Reelin activates SRC family tyrosine kinases in neurons. Curr. Biol. 13(1):18-26 . 2. Budagian, V., et al. (2003) Signaling through P2X7 receptor in human T cells involves p56lck, MAP kinases, and transcription factors AP- 1 and NF-kappa B. J. Biol. Chem. 278(3):1549-1560. 3. Gil-Henn, H. and A. Elson (2003) Tyrosine phosphatase-epsilon activates Src and supports the transformed phenotype of Neu-induced mammary tumor cells. J. Biol. Chem. 278(18):15579-15586. 4. Huang, S., et al. (2003) Defective activation of c-Src in cystic fibrosis airway epithelial cells results in loss of tumor necrosis factor-alphainduced gap junction regulation. J. Biol. Chem. 278(10):8326-8332 (cites the use of this antibody). 5. Ma, X.H., et al. (2003) Muscarinic potentiation of GABA(A) receptor currents is gated by insulin signaling in the prefrontal cortex. J. Neurosci. 23(4):1159-1168 (cites the use of this antibody). 6. Pankov, R., et al. (2003) Specific beta1 integrin site selectively regulates Akt/protein kinase B signaling via local activation of protein phosphatase 2A. J. Biol. Chem. 278(20):18671-18681. 7. Piiper, A., et al. (2003) Cholecystokinin stimulates extracellular signal-regulated kinase through activation of the epidermal growth factor receptor, Yes, and protein kinase C. Signal amplification at the level of Raf by activation of protein kinase Cepsilon. J. Biol. Chem. 278(9):7065-7072 (cites the use of this antibody). 8. Cary, L.A., et al. (2002) SRC catalytic but not scaffolding function is needed for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading. Mol. Cell. Biol. 22(8):2427-2440. 9. Cheng, J.J., et al. (2002) Cyclic strain activates redox-sensitive proline-rich tyrosine kinase 2 (PYK2) in endothelial cells. J. Biol. Chem. 277(50):48152-48157. 10. Obergfell, A., et al. (2002) Coordinate interactions of Csk, Src, and Syk kinases with aIIbb3 initiate integrin signaling to the cytoskeleton. J. Cell Biol. 157(2):265-275. 11. Song, C., et al. (2002) Molecular conformation dictates signaling module formation: example of PKCepsilon and Src tyrosine kinase Am. J. Physiol. Heart Circ. Physiol. 282(3):H1166-H1171 (cites the use of this antibody in immunohistochemistry). 12. Hauck, C.R., et al. (2001) Inhibition of focal adhesion kinase expression or activity disrupts epidermal growth factor-stimulated signaling promoting the migration of invasive human carcinoma cells. Cancer Res. 61(19):7079-7090. 13. Suter, D.M. and P. Forscher (2001) Transmission of growth cone traction force through apCAM-cytoskeletal linkages is regulated by Src family tyrosine kinase activity. J. Cell Biol. 155(3):427-438 (cites the use of this antibody in immunocytochemistry). 14. Ushio-Fukai, M., et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21(4):489-495. 15. Sussman, M.A., et al. (2000) Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1. J. Clin. Invest. 105(7):875-886

Additional Info

Recognizes human Src when phosphorylated at Tyr418. Species crossreactivity: mouse, and chicken. Species sequence homology: Other species (100%). Also reacts with Fyn and Yes.