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Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)

Cat no: S6504-36B

Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)

The Src family of protein tyrosine kinases is important in the regulation of growth and differentiation of eukaryotic cells (1,2). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (3,4).\n\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry (Paraffin): 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S6504-36B

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Brown, M.T. and Cooper, J.A. (1996) Biochim. Biophys. Acta. 1287, 121

Additional Info

Recognizes human Src only when phosphorylated at Tyr527. It may crossreact with other Src family members Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Crossreactivity: Mouse and rat.

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