Src, Recombinant, Human (pp60Src, pp60c-Src)

Src is a member of the Src family of protein tyrosine kinases. This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets. Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins. Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor. In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system. It has also been reported that mice deficient in Src develop severe osteoporosis.\n\nA total of eight membrane-associated protein tyrosine kinases have been identified within the Src gene family. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr whose roles include regulation of cytoskeletal organization, cell-cell contact, DNA synthesis, proliferation and differentiation of eukaryotic cells. Elevated activity of the proto-oncogene Src has been found in many human cancers, most notably breast cancer.The highest levels of expression of the prototype member of this gene family, Src p60, are observed in platelets and in neuronal tissues. With the fact that platelets have constitutively high levels of phosphotyrosine-containing proteins suggest that Src p60 may be important for platelet homeostasis.\n\nSource: Human recombinant enzyme expressed in Sf9\n\nSrc Kinase Assay: 20units of Src Kinase transferred 0.87pmoles phosphate/min/unit to Src kinase substrate peptide.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 9 months.

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SPECIFICATIONS

Catalog Number

S6503

Size

75U

Form

Supplied as a frozen solution in 25mM HEPES, pH 7.4, 40mM sodium chloride, 0.05mM EDTA, 10% glycerol, 0.1% NP-40, 1mM dithiothreitol.

Purity

15 units gives a major band at approximately 60kD on a silver-stained SDS polyacrylamide gel. Purified by sequential chromatography on hydroxyapatite and affinity columns. Essentially free of other protein kinase contamination.

References

Review Paper: Courtneidge, S.A., Semin. Cancer Biol. 5: 239, 1994.