Src (Tyr416) (pp60Src, pp60c-Src)

Supplier:

United States Biological

Cat no: S6504-36A

Src (Tyr416) (pp60Src, pp60c-Src)

The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are important in the regulation of growth and differentiation of eukaryotic cell. Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of tyrosine 416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of tyrosine 527 in the C-terminal tail by Csk renders the enzyme less active.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S6504-36A

Size

100ul

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13,513

Additional Info

Recognizes endogenous levels of human Src only when tyrosine 416 is dephosphorylated at ~60kD. Recognizes other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites. Species Crossreactivity: Mmuse and rat. Species Sequence Homology: chicken and Xenopus, 100%

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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