SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)

SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. SREBPs may also play some role in the apoptotic processes. SREBP1 is an ~122kD integral membrane protein that moves from ER to golgi for processing. The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi. This is the ~65kD mature form that is detected by this antibody. The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65kD protein\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: ~65kD (mature form)\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman liver lysate\n\nCellular Localization: \nIntegral membrane protein that moves from ER to Golgi in absence of sterols.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S6505-15B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in Tris-glycine, 150mM NaCl , pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by peptide immunoaffinity chromatography.

Additional Info

Recognizes human SREPB-1. Species Crossreactivity: mouse, rat, and hamster. Predicted to react with porcine based on 100% sequence homology. Immunogen sequence has 94% homology to bovine, 88% homology to Zebrafish and 70% homology to chicken.