Stabilin 2 (FEEL-2, HARE) (Biotin)

Stabilin-2, also known as HARE (hyaluronan receptor for endocytosis) and FEEL-2, is a type I transmembrane multi-domain protein that is the closest homolog of Stabilin-1. Its large extracellular domain contains seven fasciclin, multiple EGF-like, laminin type EGF-like, and a link domain. The short cytoplasmic tail of Stabilin-2 lacks the CGA interactive motif found in Stabilin-1. Instead, it contains a motif for the classical cargo adaptor, AP2. Stabilin-2 is a scavenger receptor that is expressed in the sinusoidal endothelial cells of liver, spleen, and lymph nodes. It binds and mediates the systemic clearance of hyaluronan, AGE, collagen N-terminal propeptides and acetylated LDL. By proteolytic cleavage, two isoforms (315 kD and 190 kD) with similar activities can be generated. Over the region used as immunogen, human and mouse Stabilin-2 share 86% amino acid sequence identity.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Stabilin-2. The detection limit for rhStabilin-2 is approximately 10 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 1% cross-reactivity with rhStabilin-1. \nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7950-60B

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.

P Type

Pab

Purity

Purified by human Stabilin-2 affinity chromatography and then biotinylated.

Isotype

IgG

Additional Info

Selected for use as a detection antibody in human Stabilin-2 Western Blots.