STAT 1a (Signal Transducer and Activator of Transcription 1a)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2). STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1) (3). STAT1 has two forms, the 91kD STAT1a and the 84kD STAT1b, which are encoded by the same gene with splicing variant (4). \n\nThis polyclonal antibody can be used for Western blot analysis of STAT1a expression at 1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 ug per sample. The antibody is human and mouse reactive. Whole cell lysate from HeLa, Jurkat, or A431 cells can be used as positive control and a 91kD band can be detected. No reaction to the 84kD STAT1b.

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SPECIFICATIONS

Catalog Number

S7969

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified IgG

Isotype

IgG

References

1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol 1998;16: 293-322.\n2. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 1995;64: 621-51. 3. Darnell JE Jr. STATs and gene regulation. Science 1997;277: 1630-5.\n4. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr. Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. Proc Natl Acad Sci USA 1992;89: 7836-

Additional Info

A 91kD band can be detected. No reaction to the 84kD STAT1b. Human, mouse and rat reactive.