STAT 1a (Signal Transducer and Activator of Transcription 1a)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated in response to a large number of extracellular signals including cytokines, interferons, and growth factors. All seven STATs (STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, and STAT6), STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile (3,4). STAT1 is activated by many different ligands including the IFN family (IFN-alpha, IFN-beta, IFN-gama and IL-10), the gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF01) (3). STAT1 has two forms, the 91kD STAT1alpha and the 84kD STAT1beta, which are encoded by the same gene with splicing vari-ant (1). After tyrosine phosphorylation by Janus tyrosine kinase (JAK), STATs enter the nucleus to regulate transcription of many different genes.\n\nApplications: \nWestern Blot (ECL): 0.5ug/mL \nImmunoprecipitation: 2-4ug/sample \nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nHeLa Cell Lysate \nRat Brain Tissue Extract F

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SPECIFICATIONS

Catalog Number

S7969-02

Size

25ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.2, containing 0.02% sodium azide. Supplied as an IgG fraction.

P Type

Pab

Purity

Chromatographically purified

Isotype

IgG

References

1. Schindler, C., Fu, X.Y., Improta, T., Aebersold, R. and Darnell, J.E. Jr. \n(1992) Proc. Natl. Acad. Sci. USA 89: 7836-7839. \n2. Darnell, J.E. Jr. (1997) Science 277: 1630-1635. \n3. Leonard, W.J. and O

Additional Info

Detects a ~97kD protein, correspond-ing to the apparent molecular mass of STAT1alpha on SDS-PAGE immunoblots, in human, mouse, rat and bovine cell lysates and tissue extracts. This antibody does not detect the 84kD STAT1beta.