STAT 4 (Signal Transducer and Activator of Transcription 4)

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT 1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin-12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. Perhaps not surprisingly, STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study has suggested that tyrosine and serine phosphorylation of STAT4 can be induced by both IL-12 and IFN-alpha. \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml \nImmunoprecipitation: 5ug \nWestern Blot: 1-2ug/ml \nGel Mobility Shift Assay: 1-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50 % glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-03F

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2a,k

References

1. Yakamoto, K., et al. Mol. Cell. Biol. 14: 4342-4349 (1994). 2. Zong, Z., et al. PNAS USA 91: 4806-4810 (1994). 3. Thierfelder, W.E., et al. Nature 382: 171-174 (1996). 4. Yu, C.R., et al. J. Immunol. 157: 126-137 (1996). 5. Cho, S.S., et al. J. Immunol. 157: 4781-4789 (1996). 6. Kaplan, M.H., et al. Nature 382: 174-177 (1996).

Additional Info

Reacts specifically with mouse STAT4 protein. Crossreactivity with other STAT family proteins has not been detected.