Stat 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. \n\nA variety of cytokines and growth factors act through an induction of gene expression mediated by a family of latent transcription factors called STAT (Signal Transducers and Activators of Transcription) proteins. Cytokines, hormones and growth factors proceed through a sequence of steps that includes binding to receptors in the cytokine receptor superfamily followed by the activation of the JAK family of tyrosine kinases, which results in the activation of STAT proteins. The activated STATs then become dimerized and translocated into the nucleus where they initiate specific gene transcription. STAT 5 regulates growth, differentiation, and survival of mammary, hematopoietic cells, hematopoietic malignancies, or carcinogenesis. Constitutive activation of STAT 5 may contribute to tumorigenesis, thus dephosphorylation of STAT 5 may serve as a therapeutic strategy.\n\nApplications:\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:200-1:1000\nImmunocytochemistry: 1:50\nFlow Cytometry: 1:200-1:400\nELISA: 1:10000 proposed\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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