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STAT 5a (Signal Transducer and Activator of Transcription 5a)

Cat no: S7972-10

STAT 5a (Signal Transducer and Activator of Transcription 5a)

STAT (signal transducers and activators of transcription) can be selectively phosphorylated on tyrosine and serine residues. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, thought to be essential for their selective activity. STAT5 can be activated by a variety of different agents including prolactin, IL-2, IL-3, IL-7, IL-5, growth hormone and erythropoietin. The highly homologous STAT5A and STAT5B proteins are essential mediators of prolactin and growth hormone effects and have been implicated in physiological functions including cytokine control of apoptosis, growth, reproduction, lactation, immune function, differentiation and somatic growth. \n\nApplications: Immunoblot Analysis: A 1:2000 dilution detected STAT5A in RIPA lysates from Nb2-SP cells. \n\nImmunoprecipitation: 4ul immunoprecipitated STAT5A from 500ug of Nb2-SP RIPA lysate. Immunoblot Analysis: Nb2-SP cell lysate (20ug) was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-STAT5A (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. \n\nImmunoprecipitation/Immunoblot Analysis: STAT5A was immunoprecipitated from Nb2-SP cell lysate (500ug) using anti-STAT5A (4ul). The reduced immuno-complex was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT5A (1:2000), lane 2, or the secondary antibody alone, lane 1. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilum-inescence detection system.

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SPECIFICATIONS

Catalog Number

S7972-10

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Rabbit antiserum

P Type

Pab

Purity

Neat serum

Isotype

IgG

References

Application 1. Kirken, R.A., et al., J. Biol. Chem. 272: 14098

Additional Info

Recognizes and specific for mouse STAT5A at Mr ~ 94kDa. Species crossreactivity: rat and human.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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