STAT 5a (Signal Transducer and Activator of Transcription 5a)

STATs were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play essential roles in interferon IFNa-and IFNg-regulated gene expression (1). Following the identification of these first two family members, five additional mammalian STAT proteins were cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6 (1,2). All STAT proteins share several conserved structural and functional domains: (1) conserved amino terminal domain (2) DNA binding domain (3) SH3-like region (4) conserved SH2 domain responsible for-recruitment to the receptor interaction with JAKs STAT dimerization (5) conserved tyrosine residue whose phosphorylation is required for dimerization and DNA binding (6) carboxy terminal activation domain Y= site of activating tyrosine phosphorylation. In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, specific STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding resulting in the transcriptional activation of distinct target genes(1,2). At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, a striking specificity of STAT activation is seen in response to individual cytokines(1). STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin stimulated mammary tissue and from IL-3 stimulated myeloid cells (1,2,3,4).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nImmunoprecipitation: 5ug \nWestern Blot: 1-3ug/ml \nGel Mobility Shift Assay: 1-3ug \nOptimal dilutions to be determined by the researcher.\n\nSource: Mouse ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-10D

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Schindler, C., and Darnell, J.E. Jr. Ann Rev. Biochem. 64:621

Additional Info

Reacts specifically with the STAT5a protein. Does not crossreact with the highly related STAT5b protein. Crossreactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by Western Blot using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells. Species Crossreactivity: Human, mouse and rat.