STAT 5b (Signal Transducer and Activator of Transcription 5b)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2). Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion (3, 4). Stat5b may also act as a transcriptional inhibitor as demonstrated by inhibition of NF-kB mediated signaling (5). This Stat5b-mediated inhibitory effect on NF-kB signaling does not depend on Stat5b-DNA interactions but requires the carboxyl terminus of Stat5b as well as Stat5b nuclear translocation and/or accumulation, suggesting that Stat5b is competing for a nuclear factor(s) necessary for NF-kB-mediated activation of target promoters. \n\nApplications: \nSuitable for the Western blot. Jurkat cell lysate can be used as positive control and protein band of approximate molecular weight of 92kD is detected. Western blot analysis of Stat5b in 10ug of Jurkat cell lysate using 1 ug/m antibody. Immunoreaction was detected by chemiluminescence \n\nRecommended Dilution: \nWestern blot: 1-2ug/ml\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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SPECIFICATIONS

Catalog Number

S7972-15G

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological \nimplications. Annu Rev Immunol 16:293-322 (1998). \n2. Schindler C, Darnell JE Jr. Transcriptional responses to \npolypeptide ligands: the JAK-STAT pathway. Annu Rev \nBiochem 64:621-51 (1995). \n3. Darnell JE Jr. STATs and gene regulation. Science \n277:1630-1635 (1997). \n4. Sexl V, Piekorz R, Moriggl R, Roherer J, et al. Stat5a/b \ncontribute to interleukin 7-induced B-cell precursor \nexpansion, but bcr/abl-induced transformation are \nindependent of stat5. Blood 96: 2277-2283 (2000). \n5. Luo G and Yu-Lee L. Stat5b inhibits NF-kB mediated \nsignaling. Mol Endocrinol 14: 114-123 (2000).

Additional Info

Recognizes human STAT 5b (Signal Transducers and Activators of Transcription 5b)