STAT6 is a 119kD transcription factor protein, activated by cytokines, particularly interleukin-4 and IL13. (STAT6-/-) were found to be deficient in IL-4- mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T- cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. Stat6 is critically involved in Th2 immune response.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml for 2h
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry: 1:150 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive Control:
Jurkat cells, tonsil, placenta
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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