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Stat 6, CT (Signal Transducer And Activator of Transcription 6, IL-4 Stat)

Cat no: S7972-42R

Stat 6, CT (Signal Transducer And Activator of Transcription 6, IL-4 Stat)

STAT6 is a 119kD transcription factor protein, activated by cytokines, particularly interleukin-4 and IL13. (STAT6-/-) were found to be deficient in IL-4- mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T- cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. Stat6 is critically involved in Th2 immune response.\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2h\nImmunoprecipitation: 2ug/mg protein lysate\nImmunohistochemistry: 1:150 for 10min at RT\nStaining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cells, tonsil, placenta\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-42R

Size

50ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human STAT6. Species Crossreactivity: mouse and rat.

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