STAT 6 phosphorylated (Y641) (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6) (BSA & Azide Free)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Stat6 is rapidly tyrosine phosphorylated following stimulation of appropriate cell lines with IL-3, IL-4, epidermal growth factor (EGF), but is not detectably phosphorylated following stimulation with IL-2, IL-12, or erythropoietin. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml\nImmunoprecipitation: 1-2ug/ml \nOptimal dilution to be determined by researcher.\n\nRecommended Positive Control:\nNIH 3T3 cells treated with either anisomycin (10ug/ml) or EGF (100ng/ml) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-26C1

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Also available with BSA and Azide. See S7972-26C.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol 16:293-322 (1998). 2. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 64:621-51 (1995). 3. Bluyssen HA and Levy DE. J Biol Chem 272: 4600-4605 (1997). 4. Darnell JE Jr. STATs and gene regulation. Science 277:1630-1635 (1997). 5. Quelle FW, Shimoda K, Thierfelder et al. Mol Cell Biol 15(6): 3336-43. (1995).

Additional Info

Recognizes human STAT 6 (Signal Transducers and Activators of Transcription 6), phosphorylated (Y641). Species Cross Reactivity: Mouse