STAT3 (Acute-phase response factor)

Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription \nfactor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member \nof the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor \nactivates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression \nof STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a \nvariety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such \nas cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at \nits amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl \nterminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell \nlines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for \ntransformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered \nglycolytic and oxidative phosphorylation activities characteristic of cancer cells.\n\nUniProt Number:\nP40763\n\nGene ID:\nSTAT3\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145229

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Akira, S., Nishio, Y., Inoue, M., Wang, X.-J., Wei, S., Matsusaka, T., Yoshida, K., Sudo, T., Naruto, \nM., Kishimoto, T. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related \ntranscription factor involved in the gp130-mediated signaling pathway. Cell 77: 63-71, 1994.\n2. Bhattacharya, S., Schindler, C. Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559, \n2003.\n3. Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I. \nO. ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Nat. Acad. \nSci. 104: 17305-17310, 2007.

Additional Info

Recognizes human, mouse and rat Stat3. No crossreactivity with other proteins.