Stat3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1). It is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4). It possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by tyrosine phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by serine phosphorylation at Ser727, apparently via the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3beta lacks the serine phosphorylation site within the C-terminal transcriptional activation domain (8).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunofluoresence, and Chromatin Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunofluorescence:(IF-IC) 1:100 Methanol Permeabilization required for IF. \nChromatin Immunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7971-01B

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA. See S7971-01B1.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75

Additional Info

Recognizes endogenous levels of mouse Stat3 only when phosphorylated at tyrosine 705. Does not appreciably crossreact with the corresponding phospho-tyrosines of other Stat proteins. Species Crossreactivity: human, rat, monkey.