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Stat3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (BSA & Azide Free)

Cat no: S7971-01FX

Stat3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (BSA & Azide Free)

Stat3 protein is a transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 binds to DNA as either a homodimer or as a heterodimer with Stat1. Stat3 interacts with APRE (acute phase response element) and SIE (c-sis inducible element). \n\nApplications:\nSuitable for use in Gel Supershift and Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 5-10ug/ml for 2hrs at RT.\nOptimal dilution determined by the researcher. \n\nPositive Control: \nJurkat cells \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7971-01FX

Size

250ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See S7971-01F

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Daino H, et al. Blood. 2000 Apr 15; 95(8):2577. 2. Frank DA. Mol Med 1999 Jul; 5(7):432

Additional Info

Recognizes human STAT 3 (Signal Transducers and Activators of Transcription 3). Species Crossreactivity: mouse and rat.

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