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STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

Cat no: S7971-02

STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases. They then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the acitvity of this protein. PIAS3 is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms have been described.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 0.5-2ug/ml\nWestern Blot: 0.2-2ug/ml\nImmunoprecipitation: 100-500ug/sample\nImmunocytochemistry: 2-100ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7971-02

Size

50ug

Applications

ELISA, FC, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human STAT 3. Species Crossreactivity: mouse and rat.

Alternative Names

Acute-Phase Response Factor, APRF, FLJ20882, MGC16063

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