STAT6, phosphorylated (Tyr641) (PE) (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.\n\nIntended Use:\nThe reagent is designed for flow cytometric applications intended to identify and quantitate cells expressing cytoplasmic forms of the protein.\n\nSample Preparation:\nIntracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e.g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended.\n\nSample Staining:\n1. Harvest cells, and wash twice in PBS by spinning at 200 x g for 7 minutes.\n2. If cells are to be surface stained, follow the staining procedure indicated by the antibody manufacturer.\nMethanol\n3. Resuspend a maximum of 5 x 105 washed (or surface stained) cells in 0.5 ml of cold 4% paraformaldehyde fixative and incubate at room temperature for 10 minutes.\nPBS (Dulbecco

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