STK3, Active, Recombinant, Human, GST-Tag (MST2, (Mammalian STE20-like Protein Kinase 2, MST-2, FLJ90748, KRS1, Serine/threonine Protein Kinase 3, Serine/threonine Protein Kinase Krs1, STE20-like Kinase MST2)

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.\n\nSource:\nRecombinant corresponding to aa2-492 from human STK3, fused to GST-tag at N-terminal, expressed in Sf9 cells via a baculovirus expression system.\n\nMolecular Weight:\n~78kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~31pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137377

Size

10ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 3mM DTT, 18mM glutathione, 10% glycerol.

Purity

Purified (~41%)

References

1. Taylor, L.K., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(19):10099-104. 2. Song, H., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(4):1431-6. 3. Kim, D., et al. (2010). PLoS One 5(3):e9616.