Strep/6xHistidine Starter Kit

Strep/ 6xHistidine Starter Kit H-PR for purification of double tag proteins. This unique Starter Kit contains all reagents essential for the native purification of a double tag protein containing Strep-tag(R) and 6xHistidine-tag. The first purification is performed on a Ni-NTA H-PR cartridge while the second purification uses a Strep-Tactin(R) high capacity H-PR cartridge, selecting for 6xHistidine-tag and Strep-tag(R), respectively.

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SPECIFICATIONS

Catalog Number

2-1117-000

Research Area

The (Twin-) Strep-tag(R)/Strep-Tactin(R) protein purification system provides the reliable one-step purification of highly pure proteins suitable for any application, including: Structural and functional investigations Crystallization for determination of 3D structure Due to the mild conditions Strep-tag(R) II recombinant proteins can also be used for: Assays involving protein-protein and protein-DNA interactions Investigating ligand-receptor interactions under physiological conditions Separating living cells for re-culturing purposes

Additional Info

The kit contains: Strep-Tactin(R) Superflow(R) high capacity H-PR cartridge, 1 x 1 ml Ni-NTA Superflow H-PR cartridge, 1 x 1 ml Control plasmid DNA, 50 ng Strep-Tactin(R) horse radish peroxidase (HRP) conjugate, 20 micro l Buffer W (Strep-Tactin(R) washing buffer), 120 ml Buffer E (Strep-Tactin(R) elution buffer), 25 ml Buffer R (Strep-Tactin(R) cartridge regeneration buffer), 120 ml Ni-NTA Lysis Buffer (Equilibration Buffer), 120 ml Ni-NTA Wash Buffer (Washing Buffer), 120 ml Ni-NTA Elution Buffer (Elution Buffer), 30 ml NiSO4*6H2O for recharging the Ni-NTA resin (optional), 0.53g Anhydrotetracycline (AHT), 200 micro g Comprehensive manual 2-1117-000 Strep/ 6xHistidine Starter Kit H-PR for purification of double tag proteins. This unique Starter Kit contains all reagents essential for the native purification of a double tag protein containing Strep-tag(R) and 6xHistidine-tag. The first purification is performed on a Ni-NTA H-PR cartridge while the second purification uses a Strep-Tactin(R) high capacity H-PR cartridge, selecting for 6xHistidine-tag and Strep-tag(R), respectively. The kit contains: Strep-Tactin(R) Superflow(R) high capacity H-PR cartridge, 1 x 1 ml Ni-NTA Superflow H-PR cartridge, 1 x 1 ml Control plasmid DNA, 50 ng Strep-Tactin(R) horse radish peroxidase (HRP) conjugate, 20 micro l Buffer W (Strep-Tactin(R) washing buffer), 120 ml Buffer E (Strep-Tactin(R) elution buffer), 25 ml Buffer R (Strep-Tactin(R) cartridge regeneration buffer), 120 ml Ni-NTA Lysis Buffer (Equilibration Buffer), 120 ml Ni-NTA Wash Buffer (Washing Buffer), 120 ml Ni-NTA Elution Buffer (Elution Buffer), 30 ml NiSO4*6H2O for recharging the Ni-NTA resin (optional), 0.53g Anhydrotetracycline (AHT), 200 micro g Comprehensive manual