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Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Cat no: S7975-11H


Supplier: United States Biological
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Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.01ug/ml, observed in lysates of cell line HeLa on ~150kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7975-11H
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Lara-Pezzi E, Pezzi N, Prieto I, Barthelemy I, Carreiro C, Martinez A, Maldonado-Rodriguez A, Lopez-Cabrera M, Barbero JL. Evidence of a transcriptional co-activator function of cohesin STAG/SA/Scc3. J Biol Chem. 2004 Feb 20;279(8):6553-9. Epub 2003 Dec 2.
Additional info: Recognizes human STAG2. Species sequence homology: Canine, mouse and rat.

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