Stromal Interaction Molecule 1 (STIM1, D11S4896E, GOK)

STIM1 (Stromal Interaction Molecule 1; GOK) is an essential and conserved component of store-operated Ca2+ channel function. It is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane (STIM1 translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium). STIM1 is a typical transmembrane protein with an extracellular N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that likely represents the transmembrane region. The mouse STIM1 homologue (Stim1) was identified independently as a stromal-cell-derived gene product that binds to the surface of haematopoietic cells, particularly pre-B lymphoid cells, and can promote subsequent clonal expansion. Murine Stim1 is 96% identical with human STIM1 in amino acid sequence, and maps to a region of mouse chromosome 7 syntenic with human 11p15.5.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: Reducing and non-reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRBL rat basophilic leukemia cell line\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7975-88A3

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA.: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85. 2. Draber P, Draberova L.: Lifting the fog in store-operated Ca2+ entry. Trends Immunol. 2005 Dec;26(12):621-4.

Additional Info

Recognizes mouse and rat STIM1, a 84kD essential and conserved component of store-operated Ca2+ channel function.