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SULT2A, CT (Bile Salt Sulfotransferase, Hydroxysteroid Sulfotransferase, HST, Dehydroepiandrosterone Sulfotransferase, DHEA-ST, Sulfotransferase 2A1, ST2A1, ST2 ,ST2A3, STD) (APC)

Cat no: S8022-02A-APC

SULT2A, CT (Bile Salt Sulfotransferase, Hydroxysteroid Sulfotransferase, HST, Dehydroepiandrosterone Sulfotransferase, DHEA-ST, Sulfotransferase 2A1, ST2A1, ST2 ,ST2A3, STD) (APC)

One of the major roles of the sulfotransferases (ST) in the metabolism of drugs and endogenous compounds is the conversion of these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. Sulfation may also play a regulatory role for many endogenous compounds, such as steroids and neurotransmitters, by altering the biologic properties of these compounds. Otterness et al. (1992), Kong et al. (1992), and Comer et al. (1993) reported the cloning of cDNAs encoding liver dehydroepiandrosterone (DHEA) sulfotransferase. The predicted protein has 285 amino acids. Although Northern blot analysis of human liver RNA detected transcripts of 3 different sizes, Southern blot analysis of human DNA suggested that only 1 gene is present in the genome. This gene has an important role in the sulfation of both bile acids and steroids in the liver and adrenals. The human adrenal form of this enzyme is physically, immunologically, and kinetically similar, perhaps identical, to the liver form. Dehydroepiandrosterone sulfate is quantitatively one of the major steroids secreted from the adrenal cortex.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8022-02A-APC

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Otterness, D. M., et al. Molec. Pharm. 41: 865-872 (1992). 2.Kong, A.-N. T., et al. Biochem. Biophys. Res. Commun. 187: 448-454 (1992). 3.Comer, K. A., et al. Biochem. J. 289: 233-240 (1993).

Additional Info

Recognizes human SULT2A. Species Crossreactivity: mouse.

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