SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme)

SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100,000-1:125,000 \nWestern Blot: 1:750-1:1,000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8025-85F

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Wrighton KH,and Feng XH. (2006). Uba2. AfCS-Nature Molecule Pages. doi:10.1038/mp.a003681.01 \nLois,L.M. and Lima,C.D. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. 2.EMBO J. 24 (3), 439-451 Desterro,J.M., Rodriguez,M.S., Kemp,G.D. and Hay,R.T. (1999) Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 274 (15), 10618-10624. 3.Gong,L., Li,B., Millas,S. and Yeh,E.T. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448 (1), 185-189.

Additional Info

Recognizes human. Species Sequence Homology: bovine, dog, chimpanzee(96%), mouse (93%), and rat(92%).