SUMO1, Recombinant, Human (Small Ubiquitin-related Modifier 1, SUMO-1, GAP-modifying Protein 1, GMP1, SMT3 Homolog 3, Sentrin, Ubiquitin-homology Domain Protein PIC1, Ubiquitin-like Protein SMT3C, Smt3C, Ubiquitin-like Protein UBL1, SMT3C, SMT3H3, UBL1, O

The small ubiquitin-related modifier (SUMO) belongs to the growing family of ubiquitin-related proteins involved in posttranslational protein modification. It is present in all eukaryotic kingdoms and is highly conserved from yeast to humans. The SUMO family are composed of three types; SUMO1, SUMO2 and SUMO3. Like ubiquitin, SUMO1 is conjugated to target proteins by an enzymatic cascade involving E1, E2 enzyme and E3 lygase (known as sumoylation). It has been known that many substrates such as RanGAP1, PML and IkBa proteins are present in cells. In contrast to ubiquitination, sumoylation does not tag proteins for degradation by the 26S proteosome. Sumoylation participates in diverse events such as nuclear transport, transcriptional regulation, chromosome segregation and cell cycle control. Also the modification is associated with pathological conditions (neurodegeneration, diabetes and Inflammation).\n\nSource:\nRecombinant corresponding to aa1-97, with additional 3aa-GSH at N-terminal, from human SUMO1, expressed in E.coli.\n\nMolecular Weight:\n~11.7kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0750-01T

Size

500ug

Form

Supplied as a lyophilized powder from 20mM Tris, pH 8, 10mM sodium chloride.

Purity

~95% (SDS-PAGE)

References

1. Muller S. et al., 2001, Nat Rev Mol Cell Biol. 2:202-210. 2. Gill G, 2004, Genes Dev. 18(17):2046-2059. 3. Hay RT, 2005, Mol Cell. 18(1):1-12. 4. Degerny C et al., 2005, J Biol Chem. 280(26):24330-24338.