Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)

Cu/Zn containing superoxide dismutase (SOD) belongs to the family of related enzymes which have the same catalytic function but are structurally different. SOD catalyzes the dismutation of the superoxide radical O2-to O2 and H2O2 which in turn is metabolized to H2O and O2 by catalase and glutathione peroxidase (1, 2). The SOD family is classified into subfamilies on the basis of their metal cofactors which could be either a dinuclear Cu/Zn or mononuclear Fe, Mn or Ni. The Fe-and Mn-SODs have identical metal chelating residues at the active site and share substantial sequence and tertiary homology, whereas the other SODs are structurally unrelated. The Cu/Zn-SOD is found in the cytosol of eukaryotes, in chloroplasts and in the periplasm of some prokaryotes. The eukaryotic, Cu/Zn-SODs usually consists of two identical subunits of 16kD each that are linked by two cysteines forming an intrasubunit disulfide bridge and each subunit contains a metal cluster at the active site. The Cu/Zn-SOD is believed to play a major role in antioxidant defence mechanisms (3,5). Fe-SOD is present in both aerobic and anaerobic bacteria, Archaea and plants whereas Mn-SOD is present in bacteria, Archaea, mitochondria and chloroplasts. In animals, Mn-SOD is present predominately in the mitochondria as a tetramer of 80kD (3,4). SODs are involved in maintaining the cell integrity and protecting the functionality of the single cell components against the damages by oxidative stress (5).\n \nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested\n\nRecommended Dilution:\nWestern Blot (ECL): 1ug/ml \nOptimal dilutions to be determined by researcher.\n \nNote:\nThis antibody is recommended for use with samples of mouse or rat origin.\n \nPositive Controls:\nRat Brain Tissue Extract and Mouse Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8060-09A

Size

25ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in 0.1M PBS, pH7.0, 0.1% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bannister, J. V., Bannister, W. H. and Rotilio, G. (1987) Crit. Rev. Biochem. 22: 111-\n180. \n2. Hassan, H. M. (1988) Free Radical Biol. Med. 5: 377-385. \n3. Kurobe, N., Suzuki, F., Kato, K. and Sato, T. (1990) Biomedical Research 11: 187-194. \n4. Kurobe, N. and Kato, K (1991) Biomedical Research 12: 97-103. \n5. Cannio, R., Fiorentino, G., Morama, A., Rossi, M and Bartolucci, S. (2000) Frontiers in \nBioscience 5: d768-779 \n6. Xiao-Hong Liu, et al., (1993) Brain Res. 625: 29-37 \n7. Pedraza-Chaverri, J. et al (2000) Free Radical Biology & Medicine 29(7): 602-611. \n8. Katsuki, H. et al (2001) J. Neurochem. 76: 1336-1345. \n9. Shinder, G.A., Lacourse, M.-C., Minotti, S. and Durham, H.D. (2001). J. Biol. Chem. \n276(16): 12791-12796. \n

Additional Info

This immunoaffinity purified antibody detects ~23kD (human) and 19kD (other species) proteins, corresponding to the apparent molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots.\nSpecies Crossreactivity: Human, mouse, rat and bovine