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SUR1 (ABC36, Abcc8, ATP binding cassette sub family C member 8, HHF1, HRINS, MRP8, PHHI, SUR)

Cat no: 167604

SUR1 (ABC36, Abcc8, ATP binding cassette sub family C member 8, HHF1, HRINS, MRP8, PHHI, SUR)

Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of SUR1 in 20ug of mouse brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1ug/ml\nImmunohistochemistry: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167604

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042. 2.

Additional Info

Recognizes rat SUR1. Species Crossreactivity: Mouse, Hamster, Rat. Detects ~160kD. Does not cross-react with SUR2B

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