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SUR2A (Sulfonylurea receptor 2 antibody, CMD10, ABC37.)

Cat no: 167606

SUR2A (Sulfonylurea receptor 2 antibody, CMD10, ABC37.)

Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four K\nir6.x and four SUR subunits form an ion conducting channel commonly referred to as the K\nATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir\n6.x potassium channel. Hence the K ATP channel monitors the energy balance within the cell (2).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of SUR2A in 20ug of mouse brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167606

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042.\n2. Nichols C.G. (2006) Nature. 440 (7083): 470-476.

Additional Info

Recognizes mouse SUR2A. Species Crossreactivity: Rat, Mouse. Detects ~120kD. Does not cross-react with SUR2B

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