Survivin (BIRC5, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), survivin, apoptosis inhibitor 4)

Alternate Name: BIRC5, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), survivin, apoptosis inhibitor 4\n\nDomain Information: \n\nGene Ontology Terms: 3-oxo-5-alpha-steroid 4-dehydrogenase activity; DNA repair; androgen metabolism; cell-cell signaling; electron transporter activity; integral to membrane; male gonad development; methylated-DNA-[protein]-cysteine S-methyltransferase activity; microsome; oxidoreductase activity; sex determination; sex differentiation\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 18kD band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kD according to NP_001159.2 and 18.6kD according to NP_001012271.1). Recommended concentration: 0.01-0.5ug/ml.\n\n0.01ug/ml staining of Molt-4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See S8500-06N-P. Antibody

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SPECIFICATIONS

Catalog Number

S8500-06N

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. , Control of apoptosis and mitotic spindle checkpoint by survivin., Nature. 1998 Dec 10;396(6711):580-4.

Additional Info

This antibody is expected to recognize the reported isoforms 1 and 3 (NP_001159.2 and NP_001012271.1 resp.)