Syk (Tyrosine-protein Kinase SYK)

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCy1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137496

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Cheng, A.M. and Chan, A.C. (1997) Curr. Opin. Immunol. 9, 528-533. 2. Kurosaki, T. et al. (1997) Curr. Opin. Immunol. 9, 309-318. 3. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180. 4. Turner, M. et al. (2000) Immunol. Today 21, 148-154. 5. Coopman, P.J. et al. (2000) Nature 406, 742-747. 6. Deckert, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. 7. Rao, N. et al. (2001) EMBO J. 20, 7085-7095. 8. Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315. 9. Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.

Additional Info

Recognizes endogenous levels of total human Syk. Species Crossreactivity: mouse, rat.