$Synapsin I, phosphorylated (Ser603)\n$

Supplier:

United States Biological

Cat no: S9104-04C

Synapsin I, phosphorylated (Ser603)\n

Synapsin I is a neuron-specific phosphoprotein which is associated with synaptic vesicles in the central and peripheral nervous system. Synapsin I is involved in the regulation of synaptic vesicle availablility, and is thought to play a key role in brain plasticity. Synapsin I is phosphorylated at serine 603 by CaMKII. The specificity of this antibody has been shown by blocking studies in Western blotting, whereby staining was blocked by the phosphopeptide. No blocking was seen by the corresponding non-phosphopeptide.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution: \nWestern Blot:1:1000, detects a band of approximately 78kD in rat brain lysates.\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Reagents: \nSheep anti rabbit IgG:HRP \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9104-04C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

References

1. Jovanovic, J.N. et al. (2001).Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+ - dependent glutamate release in isolated nerve terminals. J. Neurosci. 21: 7944 - 7953. 2. Kao, H.T. et al. (2002). A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci. 5: 431 - 437. 3. Menegon, A. et al. (2000). Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals. J. Cell Sci. 113: 3573 - 3582.

Additional Info

Specific for rat Synapsin I, when phosphorylated at serine 603.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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