Synapsin I, phosphorylated (Ser9)

Synapsins, a group of at least five members (synapsins Ia, Ib, IIa, IIb and IIIa), are abundant brain proteins essential for regulating neurotransmitter release. All synapsins contain a short N-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I. Phosphorylation of synapsin N-terminal domain at serine 9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles. \nSynapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation. The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9104-03

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Greengard, P. (1987) Mol. Neurobiol. 1, 81

Additional Info

Detects endogenous levels of synapsin only when phosphorylated at serine 9. Predicted to recognize phosphorylated serine 9 of all synapsin isoforms. Species Crossreactivity: Human, rat and mouse.