Synaptophysin (CT) (Major synaptic vesicle protein p38)

Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa (1). It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for the presynaptic vesicle, and functions in synaptic vesicle exocytois, including cholesterol-dependent vesicle \nbiogeneis, regulation of SNARE assembly via interaction with VAMP/synaptobrevin, formation of the fusion pore initiating neurotransmitter release, and activation of vesicle endocytosis (2, 3). This gene has also been implicated in X linked mental retardation (4). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9108-03A2

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Johnston P.A., et al. (1989) J Biol Chem. 264:1268-1273. 2. Thiele C., et al. (2000) Nat Cell Biol. 2:42-49 3. Valtorta F., et al. (2004) Bioessays 26:445-453. 4. Tarpey P.S., et al. (2009) Nat Genet. 41(5):535-543.

Additional Info

Recognizes Immunoprecipitate of human brain.