Synaptophysin

Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells(1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a \nnumber of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is \npreferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). Data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4). \n \nApplications: \nSuitable for use in: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications have not been tested. \n \nRecommended Dilution: \nWestern Blot: 1:200,000 \nImmunohistochemistry: 1:250-500 \nImmunocytochemistry: 1:250-500 \nFlow Cytometry: 1:20 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S9108-20

Size

100ul

Applications

ELISA, FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium \nazide, 0.05% BSA and 40% Glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Ozcelik T, et al. Am J Hum Genet 47(3):551-61, 1990. 2. McMahon HT, et al. Proc Natl Acad Sci 93(10):4760-4764, 1996. 3. Reisinger C, et al. J. Neurochem 90(1):1-8, 2004. 4. Tixier-Vidal A, et al. Neuroscience 26(3):847-61, 1988.

Additional Info

Recognizes human Synaptophysin. Species Crossreactivity: mouse and rat.