Synaptopodin (SYNPO, KIAA1029)

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen): Neat\nImmunohistochemistry (Paraffin): Requires microwave treatment prior to incubation.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9500-74A

Size

5ml

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, GP

Form

Supplied as a liquid, 0.01% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Mundel, P., et al., (1991) 39:8 1047-1056. 2. Mundel, P., et al., (1995) Anat. Embryol., 192, 385-397. 3. Mundel, P., et al., (1997) J. Am. Soc. Nephrol., 8, 89708-89705. 4. Mundel, P., et al., (1997) J. Cell Biol., 139:1, 193-204. 5. Mundel, P., et al., (1997) Exp. Cell Res., 236, 248-258. 6. Kobayashi, N., et al., (1998) J. Cell Biol., 143, 1961-1970. 7. Barisoni, L., et al., (1999) J. Am. Soc. Nephrol., 10, 51-61. 8. Kihara, I., et al., Histopathology, 67(6), 537-547. 9. Asanuma, K., et al., (2005), J. Clin. Invest., 115, 1188-1198.

Additional Info

Recognizes rat synaptopodn. Reacts with differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation- dependent expression during postnatal maturation of rat brain. Differentiation- dependent expression in cultured hippocampal neurons. Species Crossreactivity: human, bovine, mouse, guinea pig and gerbil. Does not react with rabbit, frog, or chicken.