Syntrophin (dystrophin-associated protein A1, 59kD, basic component 1, 59-DAP, 59kD dystrophin-associated protein A1 basic component 1, A1B, Beta-1-syntrophin, BSYN2, DAPA1B, FLJ22442, MGC111389, SNT2, SNT2B1, Syntrophin-2, Tax interaction protein 43, TIP

The syntrophins are a family of scaffolding proteins with multiple protein interaction domains that link signaling proteins to dystrophin family members. Each of the three most characterized syntrophins (alpha, beta-1, and beta-2) contains a PDZ domain that binds a unique set of signaling proteins including kinases, ion and water channels, and neuronal nitric oxide synthase (nNOS). Despite their ability to bind dystrophin in vitro, neither g-syntrophin isoform co-localizes with dystrophin in skeletal muscle. Furthermore, g-syntrophins do not co-purify with dystrophin isolated from mouse tissue. Research suggests that the interaction of g-syntrophin with dystrophin is transient and potentially subject to regulatory mechanisms. g1-Syntrophin is highly expressed in brain and is specifically localized in hippocampal pyramidal neurons, Purkinje neurons in cerebellum, and cortical neurons. g2-Syntrophin is expressed in many tissues including skeletal muscle where it is found only in the subsynaptic space beneath the neuromuscular junction. In both neurons and muscle, g-syntrophin isoforms localize to the endoplasmic reticulum where they may form a scaffold for signaling and trafficking (Adams, 2006).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9299-95B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Rabbit

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Adams, M., et al. (2006) Experimental Cell Research. 312: 3084-3095 2. Solimena, M., et al. (2001) EMBO Journal. 20: 4013:4023 3. Froehner, S. (1984). J. Cell Bio. 99: 88-96.

Additional Info

Recognizes Syntrophin. Species Crossreactivity: Rabbit, Mouse, Rat, Human, Torpedo.