Syntrophin (Syntrophin 1, Syntrophin-1, Syntrophin alpha 1, SNTA1, 59kD Dystrophin-associated Protein A1 Acidic Component 1, Dystrophin-associated Protein A1 59kDa Acidic Component, Dystrophin-associated Protein A1, Pro-TGF-alpha Cytoplasmic Domain-intera

Syntrophin (alpha-1) is a peripheral membrane protein located at the neuromuscular junction. Syntrophin (alpha-1) is expressed at high levels in skeletal muscle and heart, and at lower levels in brain, pancreas, liver, kidney and lung. It is an adaptor protein which is thought to bind and organise the subcellular localisation of membrane proteins such as utrophin and acetylcholine receptor. Syntrophin (alpha-1) also binds phosphatidylinositol 4,5-bisphosphate, it may link various receptors to the dystrophin glycoprotein complex and the actin cytoskeleton.\n\nApplications: \nSuitable for use in Immunofluorescence, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (IC): 10ug/ml\nWestern Blot: 0.2ug/ml. Detects a band at ~58kD representing syntrophin from rat and mouse cell extracts.\nImmunohistochemistry: 1:10-1:2000\nImmunoprecipitation: 1:10-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9299-95

Size

50ug

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird

Form

Supplied as a liquid in PBS, 5% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Ort T et al. Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion. EMBO J 20:4013-23 (2001). 2. Ort T et al. The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells. Eur J Cell Biol 79:621-30 (2000). 3. Froehner SC et al. A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle. J Cell Biol 104:1633-46 (1987).

Additional Info

Recognizes the alpha1, beta1 and beta2 isoforms of human Syntrophin. Species Crossreactivity: mouse, rat, amphibians, chicken, canine and fish.