TACE, CT (TNF-alpha Converting Enzyme)

Beta-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, beta-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12kD CT membrane fragment. Alternatively, alpha-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by alpha-secretase produces a soluble N-terminal fragment, APPsa, and a 10kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gamma-secretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and Ab formation in cultured cells (1). Membrane-bound TNF-a, like APP, is transmembrane protein that can undergo TACE-mediated proteolysis to release the extracellular domain as soluble TNF-a. TACE contain an autoinhibitory domain that must be removed for activity, a proteolytic domain, a disintegrin domain, a cysteine-rich domain and a transmembrane domain.\n \nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. See cat# T0500-10A.\nWestern Blot: 1-10ug/ml using ECL.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T0500-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Buxbaum, J.D., et al., J. Biol. Chem. 273: 27,765-27,767 (1998). 2. Lammich, S., et al., PNAS 96: 3922-3927 (1999). 3. Vassar, R., et al., Science 286: 735-741 (1999). 4. Yan, R., et al., Nature 402: 533-537 (1999). 5. Sinha, S., et al., Nature 402: 537-540 (1999). 6. Hussain, I., et al., Mol. Cell Neurosci. 14: 419-427 (1999). 7. Lin, X., et al., PNAS 97: 1456-1460 (2000). 8. Black, R.A., et al., Nature 385: 729-733 (1997). 9. Moss, M.L., et al., Nature 385: 733-736 (1997).

Additional Info

Recognizes rat TACE at ~70kD.