TACI (Transmembrane Activator and CAML Interactor)

Simultaneously four different laboratories identified a a new member of the tumor necrosis factor (TNF) family. This has been named as TALL-1 (1), THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase (2), BAFF (for B cell activating factor belonging to the TNF family) (3) and BlyS (B lymphocyte stimulator) (4). Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases. The receptor for BlyS/BAFF appears to be expressed predominantly on B cells and activated T cells (5). Recently two orphan receptors TACI (6) and BCMA (7,8) have been shown to be receptors for BlyS/BAFF (6-9). TACI is expressed on B cells and signals through CAML, activating the transcription factors NF-AT, NF-kB, and AP-1. TACI is a type III transmembrane protein, as defined by the lack of a cleaved signal sequence and its N-terminal extracellular exposure. The cDNA codes for 293 amino acids with predicted molecular weight of approximately 32 kD. \n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilution: \nWestern Blot: 0.1-0.5ug/ml of TACI in 10ug/lane of Daudi lysate. A protein band of ~32 kD is detected. \nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nHuman spleen cells, Raw cells, or Daudi cells \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0294-05

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Shu HB, Hu WH, Johnson H J Leukoc Biol 65(5): 680-683 (1999). 2. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. J Biol Chem 274(23): 15978-15981 (1999). 3. Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, et al. J Exp Med 189(11): 1747-1756 (1999). 4. Moore PA, Belvedere O, Orr A, Pieri K, LaFleur DW, Feng P, Soppet D, Charters M, Gentz R, Parmelee D, Li Y, Galperina O, et al. Science 285 (5425):260-3 (1999). 5. von Buelow GU and Bram RJ. Science 278: 138-141 (1997). 6. von Buelow GU, Russell H, Copeland NG, Gilbert DJ, Jenkins NA, Bram RJ. Mamm Genome 11: 628-632 (2000). 7. Laabi Y., et al. EMBO J 11: 3897-3904 (1992). 8. Gras M-P, et al. Int. Immunol 7: 1093-1106 (1995). 9. Marsters SA, Yan M, Pitti RM, Haas PE, Dixit VM, Ashkenazi A. Curr Biol 10(13): 785-788 (2000).

Additional Info

Recognizes human TACI (Transmembrane Activator and CAML Interactor). Species crossreactivity: mouse.