TANK, CT (TRAF Family Member-associated NF-kappa-B Activator, TRAF-interacting Protein, ITRAF, I-TRAF, TRAF2)

TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-kB. This activation of NF-kB occurs through an association with the kinases IKKe and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IkB complex. This suggests that in addition to the possibility that TBK1 and IKKe activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-kB proteins. At least two isoforms of TANK are known to exist.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nDaudi Cell Lysate or Rat Spleen Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146107

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cheng G and Baltimore D. TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kB activation. Genes Dev. 1996; 10:963-73. 2. Rothe M, Xiong J, Shu HB, et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Natl. Acad. Sci. USA 1996; 93:8241-6. 3. Pomerantz JL and Baltimore D. NF-kB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J. 1999; 18:6694-704. 4. Chariot A, Leonardi A, Muller J, et al. Association of the adaptor TANK with the IkB kinase (IKK) regulator NEMO connects IKK complexes with the IKKe and TBK1 kinases. J. Biol. Chem. 2002; 277:37029-36.

Additional Info

Recognizes human TANK. Species Crossreactivity: mouse and rat.