Selective isolation of Vibrio sp. from clinical or food materials.
TCBS is prepared according to the formula of Kobayashi et al. The pH of the medium is elevated to enhance the growth of V. cholerae. Sucrose is fermented by V. cholerae and its biotype Eltor producing yellowing-brown flat colonies. Fishbein et al. reported that V. parahaemolyticus produce light bluish colonies. Enteric bacteria, including Proteus sp. present in fecal specimens are inhibited. TCBS Agar was also found useful in the isolation of V.fluvialis and V. vulnificus. Gram- positive bacteria are inhibited by the bile present in the medium. Bromthymol blue and thymol blue are pH indicators.
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